| 試劑名稱 | 配制試劑方法 | 規(guī)格 | 價格 | ||
| 1 | 1.5M Tris-HCl (pH8.8, 200ml) | Tris base | 36.34g | ¥0.00 | |
| ddH2O | 160ml | ¥0.00 | |||
| 用HCl調(diào)至pH8.8后，定容至 | 200ml | 200ml | ¥0.00 | ||
| 2 | 1.0M Tris-HCl (pH6.8, 250ml) | Tris base | 30.9g | ¥0.00 | |
| ddH2O | 200ml | ¥0.00 | |||
| 用HCl調(diào)至pH6.8后，定容至 | 250ml | 250ml | ¥0.00 | ||
| ¥0.00 | |||||
| 3 | 6*上樣Buffer | 4*Tris-HCl(pH6.8) | 7ml | ¥0.00 | |
| Glycerol (甘油) | 3ml | ¥0.00 | |||
| SDS | 1g | ¥0.00 | |||
| DTT | 0.93g | ¥0.00 | |||
| bromphenol blue | 1.2mg | ¥0.00 | |||
| H2O定容至 | 10ml | 10ml | ¥0.00 | ||
| ¥0.00 | |||||
| 4 | 10%APS(過硫酸銨） | 過硫酸銨 | 0.1g | ¥0.00 | |
| ddH2O | 1ml | 1mlX10支 | ¥0.00 | ||
| ¥0.00 | |||||
| 5 | 10%SDS | SDS | 10g | ¥0.00 | |
| 加熱至68度溶解，HCl調(diào)pH7.2 | ¥0.00 | ||||
| ddH2O定容至100ml 滅菌 | 100ml | 100ml | ¥0.00 | ||
| ¥0.00 | |||||
| 6 | 10*TBS | Tris base | 24.2g | ¥0.00 | |
| NaCl | 80g | ¥0.00 | |||
| HCl調(diào)pH7.6 | ¥0.00 | ||||
| ddH2O定容至 | 1L | 1L | ¥0.00 | ||
| ¥0.00 | |||||
| 7 | Cocktail RIPA | 1ml | 1ml | ¥0.00 | |
| ¥0.00 | |||||
| 8 | 10*Running buffer | Tris base | 30.2g | ¥0.00 | |
| 甘氨酸 | 188g | ¥0.00 | |||
| 10%SDS | 100ml | ¥0.00 | |||
| ddH2O定容至 | 1L | 1L | ¥0.00 | ||
| ¥0.00 | |||||
| 9 | 10*轉(zhuǎn)膜Buffer(不含甲醇) | Tris base | 30.3g | ¥0.00 | |
| 甘氨酸 | 144g | ¥0.00 | |||
| ddH2O定容至 | 1L | 1L | ¥0.00 | ||
| ¥0.00 | |||||
| 10 | 5XTBE | Tris base | 54g | ¥0.00 | |
| Na2EDTA.H2O | 3.72g | ¥0.00 | |||
| 硼酸 | 27.5g | ¥0.00 | |||
| ddH2O定容至 | 1L | 1L | ¥0.00 | ||
| ¥0.00 | |||||
| 11 | PMSF (100mg/ml) | PMSF | 17.4mg | ¥0.00 | |
| 異丙醇 | 1ml | 1ml | ¥0.00 | ||
| ¥0.00 | |||||
| 12 | 口腔漱口液保存液 | NaCl | 13.2g | ¥0.00 | |
| 檸檬酸鈉 | 6.6g | ¥0.00 | |||
| 氯仿 | 15mL | ¥0.00 | |||
| 伊紅溶液（HE染色的伊紅） | 3-5滴 | ¥0.00 | |||
| ddH2O定容至 | 1L | 1L | ¥0.00 | ||
| ¥0.00 | |||||
| 13 | 0.5M EDTA（pH8.0）,250ml | EDTA·2Na | 42.27g | ¥0.00 | |
| NaOH | 2g | ¥0.00 | |||
| ddH2O定容至 | 250ml | 250ml | ¥0.00 | ||
| ¥0.00 | |||||
| 14 | 1M Tris-HCl(pH8.0),1L | Tris base | 121g | ¥0.00 | |
| ddH2O | 800ml | ¥0.00 | |||
| 濃HCl調(diào)pH至8.0，后定容至 | 1L | 1L | ¥0.00 | ||
| ¥0.00 | |||||
| 15 | 糞便DNA保存液 | 1M Tris-HCl | 5 ml | ¥0.00 | |
| 0.5M EDTA | 100 ml | ¥0.00 | |||
| SDS | 2.5g | ¥0.00 | |||
| ddH2O定容至 | 500ml | 500ml | ¥0.00 | ||
| ¥0.00 | |||||
| 16 | 蛋白和封閉液防腐試劑 | ¥0.00 | |||
| 任意蛋白或封閉液 | X | ¥0.00 | |||
| 0.1% sodium azide（疊氮鈉） | 1g | ¥0.00 | |||
| 0.1%Gelatin | 1g | ¥0.00 | |||
| 用PBS定容至 | 1L | ¥0.00 | |||
| 防止細(xì)菌生長，該試劑放在4度即可。 | 1L | ¥0.00 | |||
| ¥0.00 | |||||
| 17 | 蛋白酶K緩沖液 | 1M Tris-HCl | 25ml | ¥0.00 | |
| 0.5M EDTA | 50ml | ¥0.00 | |||
| ddH2O定容至 | 500ml | 500ml | ¥0.00 | ||
| ¥0.00 | |||||
| 18 | 20mg/ml 蛋白酶K | 蛋白酶K緩沖液 溶解 | 5ml | ¥0.00 | |
| 滅菌甘油 | 5ml | ¥0.00 | |||
| 終體積 | 10mL | 10ml | ¥0.00 | ||
| ¥0.00 | |||||
| 19 | F.A.A固定液，標(biāo)準(zhǔn)固定液,萬能固定液。 | 福爾馬林(38%甲醛) | 5mL | ¥0.00 | |
| 冰醋酸 | 5mL | ¥0.00 | |||
| 70%酒精 | 90mL | ¥0.00 | |||
| 甘油 | 5mL | ¥0.00 | |||
| 適用于根,莖,葉,花藥,子房組織切片.在植物形態(tài)解剖研究上應(yīng)用極廣,此固定液最大優(yōu)點是兼有保存劑作用,對染色體的觀察效果較差。幼嫩材料用50%酒精代替70%酒精,防材料收縮;甘油(丙三醇)以防蒸發(fā)和材料變硬. | 105ml | ¥0.00 | |||
| 20 | TNT buffer | Tris base 0.1M | 12.11g | ¥0.00 | |
| NaCl 150mM | 8.77g | ¥0.00 | |||
| Triton-100 0.3% | 3ml | ¥0.00 | |||
| 可增加2mM左旋咪唑Tetramisde hydrochloride） | ¥0.00 | ||||
| 加純水 | 1000ml | ¥0.00 | |||
| 21 | 4-IPBA（TSA） | 4-IPBA 20mg/ml | 20mg | ¥0.00 | |
| 加純水 | 1ml | 1ml | ¥0.00 | ||
| 22 | Azide solution | Azide 100mM | 10ml | ¥0.00 | |
| 23 | NBT（50mg/ml）(4.5ul/ml) | NBT 50mg | 1ml | ¥0.00 | |
| 700ul DMF +300ul 水 | 1ml | ¥0.00 | |||
| 24 | BCIP (50mg/ml)(3.5ul/ml) | 50mg | 1ml | ¥0.00 | |
| DMF | 1ml | ¥0.00 | |||
| 25 | NBT/BCIP development buffer(TMN buffer) | 1M Tris pH=9.5 100ml | ¥0.00 | ||
| 5M NaCl 20ml | ¥0.00 | ||||
| 1M MgCl2 50ml | ¥0.00 | ||||
| 10% Tween-20 10ml | ¥0.00 | ||||
| 補(bǔ)水1000ml | 1000ml | ¥0.00 | |||
| ¥0.00 | |||||
| 26 | 10% PVA | 100g | 1000ml | ¥0.00 | |
| 800ml水 | ¥0.00 | ||||
| 補(bǔ)水至1000ml | 1000ml | ¥0.00 | |||
| 27 | 20XSSC(粉末 1L) | NaCl 175.3g | ¥0.00 | ||
| 檸檬酸鈉*2H2O 88.2g | 1L | ¥0.00 | |||
| ¥0.00 | |||||
| 28 | 5% Pepsin | pepsin | 1g | ¥0.00 | |
| H2O | 20ml | 20ml | ¥0.00 | ||
| 29 | 20ng/ml DAPI | DAPI+抗淬滅劑 | 1mg | ¥0.00 | |
| H2O | 50ml | 50ml | ¥0.00 | ||
| 30 | DNA probe HY buffer | DNA probe | 2.5uL | ¥0.00 | |
| 20XSSC | 1ul | ¥0.00 | |||
| 去離子甲酰胺 | 6.5uL | 10ul/反應(yīng) | ¥0.00 | ||
| 31 | NTE buffer(Stop buffer NBTBCIP) | 0.5M NaCl | ¥0.00 | ||
| 0.01M Tris | ¥0.00 | ||||
| 0.005M EDTA* Na2 | ¥0.00 | ||||
| 0.0005% Tween-20 | ¥0.00 | ||||
| 32 | 2M 甘氨酸 | 1.5g 甘氨酸 | 1.5g | ¥0.00 | |
| 純水 | 10ml | 10ml | ¥0.00 | ||
| 0.2 M Sodium Bicarbonate buffer | NaHCO3 | 168mg=0.168g | ¥0.00 | ||
| 純水 | 10ml | ¥0.00 | |||
| 調(diào)節(jié)到pH=8.5-9.5 | 10ml | ¥0.00 | |||
| 33 | 1 M Sodium Bicarbonate buffer | NaHCO3 | 84.1mg | ¥0.00 | |
| 純水 | 1000uL | ¥0.00 | |||
| 調(diào)節(jié)到pH=8.5-9.5 | 1ml | ¥0.00 | |||
| 34 | 1 M NaOH | NaOH | 100mg | ¥0.00 | |
| 純水 | 2.5ml | ¥0.00 | |||
| 用塑料容器、室溫 | 2.5ml | ¥0.00 | |||
| 35 | 1 M HCl | 純水 | 2.5ml | ¥0.00 | |
| hydrochloric acid | 91.5mg | ¥0.00 | |||
| 用塑料容器、室溫 | 2.5ml | ¥0.00 | |||
| 36 | MES (0.5 M, pH 6) | MES free acid (195.24 g/mol) | 9.762 g | ¥0.00 | |
| 10N NaOH | 1.36 | ¥0.00 | |||
| 純水補(bǔ)充到100ml | 100ml | 100ml | ¥0.00 | ||
| 37 | Sodium Borate Buffer (1 M, pH 8.5) | Boric Acid (H3BO3) (61.83 g/mol) | 6.183 g | ¥0.00 | |
| NaOH (40.00 g/mol) | 1 g | ¥0.00 | |||
| 純水 | 100ml | 100ml | ¥0.00 | ||
| 38 | MOPS Buffer (10X) (0.2 M, pH 7) | MOPS free acid (mw: 209.26 g/mol) | 4.186 g | ¥0.00 | |
| Sodium Acetate (mw: 82.03 g/mol) | 410 mg | ¥0.00 | |||
| Na2EDTA (mw: 372.24 g/mol) | 372 mg | ¥0.00 | |||
| 純水 | 100ml | 100ml | ¥0.00 | ||
| 39 | Maleic Acid (1 M, pH 7.5) | Maleic Acid (mw: 116.07 g/mol) | 11.61 g | ¥0.00 | |
| NaCl (mw: 58.44 g/mol) | 8.76 g | ¥0.00 | |||
| NaOH (mw: 40.00 g/mol) | 4 g | ¥0.00 | |||
| 純水 | 100ml | 100ml | ¥0.00 | ||
| 40 | CAPS (0.5 M, pH 10.4) | CAPS (mw: 221.32 g/mol) | 11.066 g | ¥0.00 | |
| 純水 | 100ml | 100ml | ¥0.00 | ||
| 41 | Carbonate-Bicarbonate Buffer (pH 9.5，0.1M） | Sodium bicarbonate (84.01 g/mol) | 623 mg | ¥0.00 | |
| Sodium carbonate (anhydrous) (105.99 g/mol) | 274 mg | ¥0.00 | |||
| 純水 | 100ml | 100ml | ¥0.00 | ||
| 42 | Carbonate-Bicarbonate Buffer (pH 9.2，0.2M） | Sodium bicarbonate (84.01 g/mol) | 1.529 g | ¥0.00 | |
| Sodium carbonate (anhydrous) (105.99 g/mol) | 191 mg | ¥0.00 | |||
| 純水 | 100ml | ¥0.00 | |||
| 43 | Tris Buffer (1 M, pH 7.2) | Tris base (mw: 121.14 g/mol) | 60.57 g | 100ml | ¥0.00 |
| Adjust pH using HCl pH=7.2 | ¥0.00 | ||||
| 44 | 1M DTT | DTT | 3.09g | ¥0.00 | |
| 0.01M 醋酸鈉（pH=5.2） | 20ml | ¥0.00 | |||
| 0.22uM過濾，分成2ml小份。-20保存 | 20ml | ¥0.00 | |||
| 45 | 琥珀酸溶液 | 琥珀酸試劑分子生物純度 WM：118.09 | 11.8 | ¥0.00 | |
| 純水 | 500ml | ¥0.00 | |||
| 調(diào)整到 pH=5.5 | ¥0.00 | ||||
| 定容至500ml | 500ml | ¥0.00 | |||
| 46 | Chelex溶液 | Chelex溶液 DNA提取專用 | 100ml | 100ml | ¥0.00 |
| 47 | Acetate Buffer （醋酸鈉溶液） | Sodium Acetate (mw: 82.03 g/mol) | 577 mg | ¥0.00 | |
| Acetic Acid (mw: 60.05 g/mol) | 178 mg | ¥0.00 | |||
| pH值調(diào)整到pH 5.0-5.2 | 100ml | ¥0.00 | |||
| 48 | Citrate Buffer (0.1 M, pH 6.0) | Sodium Citrate dihydrate (mw: 294.10 g/mol) | 2.427 g | ¥0.00 | |
| Citric Acid (mw: 192.12 g/mol) | 336 mg | ¥0.00 | |||
| pH值調(diào)整到pH 6.0 | 100ml | ¥0.00 | |||
| 49 | 二乙醇胺溶液 | diethanolamine (mw: 105.14 g/mol) | 10.514 g | ¥0.00 | |
| MgCl2-6H2O (mw: 203.30 g/mol) | 10 mg | ¥0.00 | |||
| pH值調(diào)整到pH 9.8 | 100ml | ¥0.00 | |||
| 50 | Imidazole-HCl Buffer (0.05 M, pH 7.0) | Imidazole (mw: 68.08 g/mol) | 340 mg | ¥0.00 | |
| HCl (mw: 36.46 g/mol) | 89 mg | 100ml | ¥0.00 | ||
| ¥0.00 | |||||
| 51 | TAE (1 M, pH 8.5，50X) |
Tris
base (mw: 121.14 g/mol) |
121g | ¥0.00 | |
| Disodium EDTA (mw: 372.24 g/mol) | 9.305g | ¥0.00 | |||
| Acetic Acid (mw: 60.05 g/mol) | 29.978g | ¥0.00 | |||
| 加水調(diào)節(jié)到500ml | 500ml | 500ml | ¥0.00 | ||
| ¥0.00 | |||||
| 52 | TE Buffer 10X | Tris-Cl (desired pH) (mw: 157.594 g/mol) | 1.576 g | ¥0.00 | |
| EDTA (pH 8) (mw: 292.24 g/mol) | 292 mg | ¥0.00 | |||
| 調(diào)整到pH7.5 或8.0 | 100ml | 100ml | ¥0.00 | ||
| 53 | 原位雜交RNA修復(fù)液 | Trisodium citrate | 29.4 | 1000ml | ¥0.00 |
| trisodium citrate | 5ml | ¥0.00 | |||
| 溶液名稱 | 組成 | 濃度 | pH值 | ¥0.00 | |
| 54 | 細(xì)胞穿透液，pH7.5 | HEPES | 15mM | ¥0.00 | |
| KCl | 50mM | ¥0.00 | |||
| MgCl2 | 1mM | ¥0.00 | |||
| EGTA | 1mM | ¥0.00 | |||
| DTT | 1mM | ¥0.00 | |||
| Saponin | 0.01% | ¥0.00 | |||
| Triton | 0.02% | pH7.5 | ¥0.00 | ||
| 55 | 0.5M EGTA | EGTA | 0.5M | ¥0.00 | |
| pH8.0 | ¥0.00 | ||||
| 緩沖液 | 使用液 | 儲備液 | ¥0.00 | ||
| 56 | Tris-乙醇（TAE） | 1×:0.04M Tris-乙酸 | 50×:242g Tris堿 | ¥0.00 | |
| 1×: 0.001M EDTA | 50×:57.1ml 冰乙酸 | ¥0.00 | |||
| 50×:100ml 0.5M EDTA(PH8.0) | ¥0.00 | ||||
| 57 | Tris-磷酸（TPE） | 1×：0.09M Tris-磷酸 | 10×：108g Tris堿 | ¥0.00 | |
| 0.002M EDTA | 15.5ml 85%冰乙酸（1.679g/ml) | ¥0.00 | |||
| 40ml 0.5M EDTA(PH8.0) | ¥0.00 | ||||
| 58 | Tris-硼酸（TBE） | 0.5×:0.045 Tris-硼酸 | 5×:54g Tirs堿 | ¥0.00 | |
| 0.5×:0.001M EDTA | 5×:27.5g硼酸 | ¥0.00 | |||
| 5×: 20ml 0.5M EDTA(PH8.0) | ¥0.00 | ||||
| 59 | 堿性緩沖液 | 1×:50mM NaOH | 1×:5ml 10mol NaOH | ¥0.00 | |
| 1×:1M EDTA | 1×:2ml 0.5M EDTA(PH8.0) | ¥0.00 | |||
| 60 | Tris-甘氨酸 | 1×:25mM Tris | 5×：15.1g Tirs堿 | ¥0.00 | |
| 1×:250mM 甘氨酸 | 94g甘氨酸（電泳級）(PH8.3) | ¥0.00 | |||
| 1×: 0.1%SDS | 50ml 10%SDS（電泳級） | ¥0.00 | |||
| 61 | HEPESS Buffer （pH= 7.5） | 配1M溶液，稱量Hepes（238.30 g/mol） | 23.83g | ¥0.00 | |
| 量取80ml純凈水，定容100ml | 100ml | ¥0.00 | |||
| ¥0.00 | |||||
| 備注：外顯子生物實驗室溶液中心，能24小時送達(dá)廣州市范圍內(nèi)實驗室，質(zhì)量保證。價格在50-150元內(nèi)。 | |||||
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